Data produced by Biolog Phenotype MicroArrays are longitudinal measurements of cellsâ respiration on distinct substrates. Biolog Phenotype Microarray Is a Tool for the Identification of Multidrug Resistance Efflux Pump Inducers. Blanco P(1), Corona F(1), Martínez JL(2). OmniLog PM software contains a suite of algorithms that work in conjunction with the OmniLog PM system and Phenotype MicroArray panels to automate incubation of up to fifty microplates at a fixed user-controlled temperature with complete collection of colorimetric assay data over time. Author(s): Joyner, Dominique | Abstract: The Biolog OmniLog? Evaluate nearly 2000 phenotypes of a microbial cell in a single experiment. Biolog Phenotype Microarray Technology: The phenotype analysis was carried out by using a new tool, Phenotype MicroArrays (PMs). ⢠Compared to other strains, C. difficile R20291, a recent hypervirulent outbreak associated strain, ⦠The present study of 1,047 different metabolic activities provides a framework for future gene-function investigations of the unique biology of the psychrophilic fungi. Phenotype MicroArrays 3938 Trust Way Hayward, CA 94545 Telephone: 510-785-2564 Fax: 510-782-4639 www.biolog.com INSTRUCTIONS FOR USE (PM Kit, Biolog Catalog # 12191) (PM1 MicroArray, Biolog Catalog # 12111) (PM2 MicroArray, Biolog Catalog # 12112) (PM3 MicroArray, Biolog Catalog # 12121) (PM4 MicroArray, Biolog Catalog # 12131) Summary: opm is an R package designed to analyse multidimensional OmniLog® phenotype microarray (PM) data. Biolog Phenotype MicroArray (PM) A total of twelve different E15.5 embryos per genotype were obtained from five separate litters, to generate neurospheres for Phenotype MicroAssay experiments. Antimicrob Agents Chemother. If the phenotype is strongly "positive" in a well, the cells actively generate energy and reduce a tetrazolium dye, forming a strong color. Biolog is a world leader in cell-based phenotypic testing technologies and assays. fischeri against antibiotics, chemicals and osmolytes using Phenotype Microarray. More Information Biolog phenotype microarrays (PMs) enable simultaneous, high throughput analysis of cell cultures in different environments. Biolog, Inc., creator of powerful cell analysis technologies for biomedical research and development, announced today a breakthrough in autism research made by using its advanced Phenotype MicroArray (PM) cell scanning technology. BIOLOG Phenotype Microarrays Investigation of the metabolism of isolated germs. Metabolic profiles of the biofilm and non-biofilms isolates were determined by phenotype microarrays (Biolog Omnilog). OmniLog PM is a dedicated system for phenotype microarray testing and includes the following components: ⢠Dedicated Windows®-based computer with preinstalled OmniLog PM Data Collection module ⢠OmniLog Incubator/Reader We introduce a three-step pipeline to analyze phenotypic microarray data with novel procedures for grouping, normalization and effect identification. Therefore, the use of a commercially available Phenotype MicroArray⢠(Biolog) in which twenty-five 96-well plates in which nearly every well had different metabolites, substrates or conditions was assessed as a way of generating phenotypic data rapidly. Data produced by Biolog Phenotype MicroArrays are longitudinal measurements of cells' respiration on distinct substrates. phenotype microarray (PM) data. Botrytis cinerea, which causes gray mold, is an important pathogen in four important economic crops, tomato, tobacco, cucumber and strawberry, in China and worldwide.Metabolic phenomics data on B. cinerea isolates from these four crops were characterized and compared for 950 phenotypes with a BIOLOG Phenotype MicroArray (PM). Tools for analysing OmniLog(R) Phenotype Microarray (PM) data as produced by the device distributed by BIOLOG Inc. and similar kinds of data such as growth curves are available in the opm package. HAYWARD, CA--(Marketwire - April 08, 2011) - Biolog, Inc. reported today that two recent scientific presentations have demonstrated the usefulness of Biolog's Phenotype MicroArray⢠technology for detecting alterations in sensitivity of cancer cells to anti-cancer drugs. The 95 different substrates of the Biolog⢠system are ideal for analyzing the biochemical properties of isolates. A phenotype microarray system enables one to monitor simultaneously the phenotypic reaction of cells to environmental challenges or exogenous compounds in a high-throughput manner. We introduce a three-step pipeline to analyze phenotypic microarray data with novel procedures for grouping, normalization and effect identification. The results indicated that this mutant was susceptible to 66 structurally unrelated compounds . Simple protocols are available for testing nearly 2000 cellular phenotypes simultaneously. Just as the molecular assay technologies have made it possible to analyse gene transcript or protein or metabolite levels in the cell, Phenotype MicroArrays make it ⦠Over 1,000 aerobes have been profiled by the Biolog OmniLog® PM. Phenotype MicroArrays for Microbial Cells Webinar Topics Include Simultaneous analysis of genomic and phenotypic data Analysis of data from a single organism, as well as mutants and multiple strains Phenotypic data analysis using the Biopython library Understanding Antibiotic Mechanism of Action with Phenotype Microarrays⢠and Machine Learning Botrytis cinerea, which causes gray mold, is an important pathogen in four important economic crops, tomato, tobacco, cucumber and strawberry, in China and worldwide.Metabolic phenomics data on B. cinerea isolates from these four crops were characterized and compared for 950 phenotypes with a BIOLOG Phenotype MicroArray (PM). Download Ebook Opm An R Package For Analysing Omnilog Phenotypededicated and customizable plots, opm provides management, visualization and statistical analysis of PM data, including curve-parameter estimation and discretization, dedicated and customizable plots, metadata management, automated generation of textual and tabular reports, ⦠Biolog Phenotype Microarray Is a Tool for the Identification of Multidrug Resistance Efflux Pump Inducers. Some of the differential gene expressions were validated by both RT-PCR and Western blot analysis. Biolog PM is a complex platform to facilitate the meta-analysis of phenomics data of microbial organisms . They use 96 well plates pre-loaded with carbon-energy and nitrogen substrates, ions, hormones/cytokines and anti-cancer agents. The output is high-density time-course data showing redox curves (approximating growth) for each experimental condition. The CSIRO researchers used Biolog Phenotype MicroArray plates to culture the fungus simultaneously in hundreds of different micro-scale culture conditions. Biologâs Phenotype MicroArrays consist of different sets of chemicals preloaded and dried into wells of 96-well MicroPlatesâ¢. The Phenotype MicroArray technology can be reliably used as a rapid screening tool for characterization in anaerobic microbial ecology. To search rapidly for metabolic differences, a working method using Biolog Phenotype MicroArray ⦠Tools for analysing OmniLog (R) Phenotype Microarray (PM) data as produced by the device distributed by BIOLOG Inc. and similar kinds of data such as growth curves are available in the opm package. Box 998, Highland Park, Winooski, Vermont 05404-0998 USA Phone: 888-451-5171 Outside the USA: 802-655-4740 The OmniLog II Combo System has additional software and a database for identification of aerobic bacteria using Biologâs Gen III microplates. MSMEG_2619 (efpA) and MSMEG_3563 mask the effect of ⦠Nurture your network and further your business goals with smart intelligence on the people and companies that matter most to you. Biolog Phenotype MicroArray data indicated that mmp deletion increased susceptibility for phleomycin, bleomycin, capreomycin, amikacin, kanamycin, cetylpyridinium chloride, and several sulfa drugs. Microarray analysis revealed differential expression of numerous genes at both early (6 h) and late (120 h) stages of infection in the resistant IET8585 cultivar over the susceptible IR24. We have focused our efforts on developing technologies and products to test properties of cells (phenotypes) very simply and efficiently. Biologâs Phenotype MicroArray⢠for Mammalian Cells (PM-M) Technology represent the fourth major technology, âPhenomicsâ, alongside Transcriptomics, Proteomics, and Metabolomics that are needed in the genomic era. The first approach is based on q ⦠A1 Negative Control A2 Positive Control A3 L-Alanine A4 L-Arginine A5 L-Asparagine A6 L-Aspartic Acid A7 L-Cysteine A8 L-Glutamic Acid A9 Adenosine-3â,5â-cyclic The use and potential of the PM system as a tool for studying niche overlap and catabolic versatility of saprotrophic fungi is discussed here, and examples of its application are provided. Abstract. Utilize preconfigured 96 well plates containing different classes of chemical compounds designed to test for the presence or absence of specific cellular phenotypes. Biologâs Phenotype MicroArray⢠Technology can be used to characterize over 1,400 phenotypes in a simple, rapid, and cost-effective manner Phenotype MicroArrays - Mammalian Mitochondrial Function Assays with MitoPlates Biolog, Inc. reported today that two recent scientific presentations have demonstrated the usefulness of Biolog's Phenotype MicroArray⢠technology for detecting alterations in sensitivity of cancer cells to anti-cancer drugs. In addition to its novel Phenotype MicroArray technology, Biolog also offers tools for the identification and characterization of microbes ranging from bacteria to fungi. opm provides management, visualization and statistical analysis of PM data, including curve-parameter estimation and discretization, Page 12/39. Neosartorya fischeri is the leading source of spoilage in thermally processed products. Using a colorimetric reaction that is indicative of respiration, these microplate assays measure the response of an individual strain or microbial community to a l ⦠We introduce a three-step pipeline to analyze phenotypic microarray data with novel procedures for grouping, normalization and effect identification. Biologâs Phenotype MicroArray technology (Biolog, USA) offers a unique way to identify natural product hits and to infer a mode of action by which the novel inhibitor (the extract) prevents microbial growth [27]. This unit has been using Biolog instruments and microplates for scientific research since 2014, to identify microbial strains against isolates of a variety of food-borne pathogenic bacteria and fungi from different sources, to carry out high-throughput phenotyping studies for important food-borne pathogenic microorganisms that the laboratory is concerned about, to obtain the phenotypic differences of ⦠Automated anti-cancer drug assays Phenotype MicroArray⢠9 Osmotic/Ionic response assays from Biolog, Inc. Reproducibility of Biolog Phenotype MicroArray data with wild type P. aeruginosa strains, PA01, and PAK. Tuberculosis is a major human and animal disease of major importance worldwide. opm provides management, visualization and statistical analysis of PM data, including curve-parameter estimation and discretization, dedicated and customizable plots, metadata management, automated generation of textual and tabular reports, ⦠The commercially available, pre-configured Biolog Phenotype MicroArrays (Biolog Inc., Harvard, CA, USA) are composed of microtiter plates with one negative control well and 95 wells pre-filled with a nutrient source (e.g., C,N,P,S, amino acids) or substrates leading to inhibitory conditions (pH, NaCl, antibiotics) in a dried state. Biolog microarrays techniques. Summary: opm is an R package designed to analyse multidimensional OmniLog® phenotype microarray (PM) data. Biolog Phenotype Microarray Is a Tool for the Identification of Multidrug Resistance Efflux Pump Inducers -The Phenotype MicroArray has been adapted to profile an iron-reducing, low biomass, obligate anaerobe. Author information: (1)Centro Nacional de Biotecnología, CSIC, Madrid, Spain. The set of culture conditions is designed to contain a diverse range of nutritional and stress conditions that a ⦠R-Forge: opm and opmdata: R Development Page Spoilage of heat processed food and beverage by heat resistant fungi (HRF) is a major problem for food industry in many countries. The BIOLOG Phenotype Microarray platform offers a wealth of possible analyses, and this paper is by no means a final analysis. For rapid automated incubation and identification of aerobic bacteria. DESCRIPTION (provided by applicant): The principal goal of this project is to adapt the "Phenotype MicroArray[TM]" ("PM") technology of Biolog, Inc (Hayward, CA) for fastidious and microaerophilic bacterial species, with special emphasis on those impo Tuberculosis is a major human and animal disease of major importance worldwide. OmniLog® phenotype microarray (PM) data. experimental data obtained using Biolog arrays (see Methods). An overlay of data from plate PM5, representing multiple replicates of assays with each of the parental strains PAK and PAO1, illustrates the degree of ⦠Genetically, the closely related strains within the Mycobacterium tuberculosis complex which cause disease are well-characterized but there is an urgent need better to understand their phenotypes. Phenotype MicroArray⢠Technology enables researchers to characterize cells in up to 1,920 assays and evaluate cell changes under thousands of culture conditions and physiological states in a simple, rapid, efficient and cost-effective manner. Examples of well components could include but are not limited to C,N,P,S-sources, amino acids, pH or NaCl tolerance, or antibiotic resistance.
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